Establishing the Roles of Multi-enzyme Complexes in Metabolic Network Regulation (Obata CAREER)

Dr. Obata’s CAREER project titled “Establishing the Roles of Multi-enzyme Complexes in Metabolic Network Regulation” is an NSF-funded project, which aimed to elucidate the principles regulating the metabolic network (research) and improve public perception of the importance of metabolism by stimulating interest in metabolism among a broad range of audiences (education). The project has the following three objectives:

-          Objective 1 (Research). Evaluate the effects of changes in multi-enzyme complex stability on metabolic flux. The rate of complex association will be artificially modified, and its effects on metabolic flux will be evaluated.

-          Objective 2 (Research). Evaluate the effects of metabolic flux changes on the multi-enzyme complex. Yeast cells will be grown under various conditions to cause flux changes on TCA cycle metabolism, and the correlation between metabolic flux and multi-enzyme complex association will be quantitatively analyzed.

-          Objective 3 (Education). Develop an interactive, hands-on learning activity to teach metabolic regulation to a wide range of audiences. This hands-on activity showing the multi-enzyme complex concept (Figure 1) will be developed together with undergraduate and graduate students. The presentation will be used in my department’s capstone course and disseminated to K-12 students and teachers as well as public audiences.

More information about the project can be found at https://news.unl.edu/newsrooms/today/article/award-bolsters-obatas-research-into-metabolic-missteps/ or https://www.nsf.gov/awardsearch/showAward?AWD_ID=1845451.

 

MERC provides evaluation support for this program through formative feedback and the assessment of impacts.

 

CONTACT

Changsoo SongRESEARCH ASSISTANT PROFESSOR

METHODOLOGY AND EVALUATION RESEARCH CORE FACILITY AT SBSRC